Validation of a model of the GAL regulatory system via robustness analysis of its bistability characteristics

In Saccharomyces cerevisiæ, structural bistability generates a bimodal expression of the galactose uptake genes (GAL) when exposed to low and high glucose concentrations. This indicates that yeast cells can decide between using either the limited amount of glucose or growing on galactose under changing environmental conditions. A crucial requirement for any plausible mechanistic model of this system is that it reproduces the robustness of the bistable response observed in vivo against inter-individual parametric variability and fluctuating environmental conditions

Red blood cell as an adaptive optofluidic microlens

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Effect of coil charge duration on combustion variability and flame morphology in a GDI engine working in lean burn conditions

Spark ignition (SI) and subsequent flame front development exert a significant influence on cyclic variability of internal combustion engines (ICEs). The increasing exploitation of lean air-fuel mixtures in SI engines to lower fuel consumption and CO2 emissions is driving the scientific community towards the search for innovative combustion strategies. Moreover, although lean combustion has been widely investigated and an important number of studies is already present in literature, the high cyclic variability typical of this combustion process still represents a major hinder to its exploitation. This study aims to investigate the effects of increasing ignition energy on combustion characteristics of lean mixtures. Tests were performed on an optically accessible gasoline direct injection (GDI) engine that allowed to investigate the correlation between the thermodynamic results and spark arc-flame morphology. Engine speed was fixed at 2000 rpm, a relative air fuel ratio (AFRrel) of about 1.3 was selected and ignition timing was set at 12 crank angle degrees (CAD) bTDC. Coil charge duration was swept from 10 to 40 CAD. Two intake pressure levels were investigated, the first corresponding to wide open throttle under naturally aspirated operating mode, the second with an intake pressure of 1.2 bar, thus corresponding to a boosted operating condition. Two dedicated scripts built using NI Vision were employed for image processing, allowing the evaluation of temporal and spatial evolution of the early stages of combustion. Arc elongation and flame front contour were used as correlation parameters that characterize flame kernel inception and development. The results confirm that, as expected, the increase of the coil charge duration tends to reduce cyclic variability in terms of engine output. The optical investigations revealed that for both examined cases the standard deviation related to the wrinkling effect on flame edge at CA5 decreased as the coil charge duration increased

Agrochemical contamination of honey and bee bread collected in the piedmont region, Italy

This study shows the results of a local biomonitoring plan developed by a regional beekeeping association, Aspromiele, in several areas of Piedmont (Italy), in order to understand the status of contamination from pesticides present in the environment and eventually to evaluate their impact on apiculture. Glyphosate was the most abundant chemical found in the bee bread and honey samples. The other pesticides detected at lower concentrations and minor frequency were mandipropamid, tau-fluvalinate, metalaxil and spiroxamine. Even if in the present study the pesticides found in the bee bread and honey were limited to a few molecules, it is important to highlight that the presence of glyphosate could represent a hazard to bees. Honeybees are the main pollinators in agricultural ecosystems, and thus appropriate environmental management could lead to a reduction in the impact of these chemicals on bees and other beneficial insects

A Novel Hierarchy of Integrable Lattices

In the framework of the reduction technique for Poisson-Nijenhuis structures, we derive a new hierarchy of integrable lattice, whose continuum limit is the AKNS hierarchy. In contrast with other differential-difference versions of the AKNS system, our hierarchy is endowed with a canonical Poisson structure and, moreover, it admits a vector generalisation. We also solve the associated spectral problem and explicity contruct action-angle variables through the r-matrix approach.Comment: Latex fil

Preliminary results of phase II study of capecitabine and gemcitabine (CAP-GEM) in patients with metastatic pretreated thymic epithelial tumors (TETs)

Background: No previous prospective trials have been reported with capecitabine and gemcitabine (CAP-GEM) in patients with metastatic thymic epithelial tumors (TETs). We conducted a multicenter study to determine the activity and tolerability of this regimen in pretreated TETs. Patients and methods: A total of 15 patients were enrolled in the first stage of phase II study. All patients received CAP-GEM every 3 weeks. The primary end point was objective response rate (RR); secondary end points were toxicity, progression-free survival (PFS) and overall survival. Results: Complete responses (CR) and partial responses were observed in three (20%) and three (20%) patients for a 40% RR, respectively. Grade 1-2 neutropenia, anemia and thrombocytopenia were the most common side-effects, noted in seven (46.7%), five (33.3%) and five (33.3%) patients, respectively. The most common grade 3 toxicity was neutropenia in three patients (20%). Median PFS was 11 months (95% confidence interval 4-17). The 1- and 2-year survival rates were 80% and 67%, respectively. Conclusion: We have decided to publish the preliminary results because this regimen was more active than that expected. Although our results are preliminary, CAP-GEM shows activity and safety in pretreated TETs. Furthermore, multicenter trials, also in first-line setting, are necessary to confirm our results

Design, Computational Modelling and Experimental Characterization of Bistable Hybrid Soft Actuators for a Controllable-Compliance Joint of an Exoskeleton Rehabilitation Robot

This paper presents the mechatronic design of a biorobotic joint with controllable compliance, for innovative applications of “assist-as-needed” robotic rehabilitation mediated by a wearable and soft exoskeleton. The soft actuation of robotic exoskeletons can provide some relevant advantages in terms of controllable compliance, adaptivity and intrinsic safety of the control performance of the robot during the interaction with the patient. Pneumatic Artificial Muscles (PAMs), which belong to the class of soft actuators, can be arranged in antagonistic configuration in order to exploit the variability of their mechanical compliance for the optimal adaptation of the robot performance during therapy. The coupling of an antagonistic configuration of PAMs with a regulation mechanism can achieve, under a customized control strategy, the optimal tuning of the mechanical compliance of the exoskeleton joint over full ranges of actuation pressure and joint rotation. This work presents a novel mechanism, for the optimal regulation of the compliance of the biorobotic joint, which is characterized by a soft and hybrid actuation exploiting the storage/release of the elastic energy by bistable Von Mises elastic trusses. The contribution from elastic Von Mises structure can improve both the mechanical response of the soft pneumatic bellows actuating the regulation mechanism and the intrinsic safety of the whole mechanism. A comprehensive set of design steps is presented here, including the optimization of the geometry of the pneumatic bellows, the fabrication process through 3D printing of the mechanism and some experimental tests devoted to the characterization of the hybrid soft actuation. The experimental tests replicated the main operating conditions of the regulation mechanism; the advantages arising from the bistable hybrid soft actuation were evaluated in terms of static and dynamic performance, e.g., pressure and force transition thresholds of the bistable mechanism, linearity and hysteresis of the actuator response